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Stain density is an important parameter for optimising the quality of ultrastructural data obtained from several types of 3D electron microscopy techniques, including serial block-face electron microscopy (SBEM), and focused ion beam scanning electron microscopy (FIB-SEM). Here, we show how some straightforward measurements in the TEM can be used to determine the stain density based on a simple expression that we derive. Numbers of stain atoms per unit volume are determined from the measured ratio of the bright-field intensities from regions of the specimen that contain both pure embedding material and the embedded biological structures of interest. The determination only requires knowledge of the section thickness, which can either be estimated from the microtome setting, or from low-dose electron tomography, and the elastic scattering cross section for the heavy atoms used to stain the specimen. The method is tested on specimens of embedded blood platelets, brain tissue and liver tissue. 相似文献
54.
Patsy V. Ramírez-González María Mercedes Zavala Arriaga Vladimir A. Escobar-Barrios 《Petroleum Science and Technology》2018,36(16):1242-1249
Interfacial tension and contact angle are two specific important parameters to take decisions for enhanced oil recovery, for instance, proper chemicals to use for surface tension reduction, expected wettability of solids, interaction between crude oil and rock. For this purpose, the article presents a method for easy calculation of the solid-liquid interfacial tension based on contact angle measurements applying Neumann's correlation and Young's equation. The main idea stands on the calculation of the rock parameters, like wettability, with known substances and extend these results to crude oils. It was possible, based on the results obtained, to establish a relationship between solid-liquid interfacial tension and contact angle for the crude oil – rock system, which can definitively be used for the calculation of interfacial tension of any other fluid spread out on the same kind of rock. A linear regression was obtained with an accuracy as good as R2 = 0.9989. Viscosity as a function of contact angle could also be obtained for the studied crude oils in the same kind of rock. 相似文献
55.
Dr. Julien J. Freudenreich Dr. Sean Bartlett Dr. Naomi S. Robertson Dr. Sarah L. Kidd Dr. Suzie Forrest Dr. Hannah F. Sore Dr. Warren R. J. D. Galloway Dr. Martin Welch Prof. David R. Spring 《ChemMedChem》2020,15(14):1289-1293
The cylindrocyclophanes are a family of macrocyclic natural products reported to exhibit antibacterial activity. Little is known about the structural basis of this activity due to the challenges associated with their synthesis or isolation. We hypothesised that structural modification of the cylindrocyclophane scaffold could streamline their synthesis without significant loss of activity. Herein, we report a divergent synthesis of the cylindrocyclophane core enabling access to symmetrical macrocycles by means of a catalytic, domino cross-metathesis-ring-closing metathesis cascade, followed by late-stage diversification. Phenotypic screening identified several novel inhibitors of methicillin-resistant Staphylococcus aureus. The most potent inhibitor has a unique tetrabrominated [7,7]paracyclophane core with no known counterpart in nature. Together these illustrate the potential of divergent synthesis using catalysis and unbiased screening methods in modern antibacterial discovery. 相似文献
56.
Multi-modal canonical correlation analysis (MCCA) is an important joint dimension reduction method and has been widely applied to clustering tasks of multi-modal data. MCCA-based clustering is usually dimension reduction of high-dimensional data followed by clustering of low-dimensional data. However, the two-stage clustering is difficult to ensure the adaptability of dimension reduction and clustering, which will affect the final clustering performance. To solve the issue, we propose a novel clustering adaptive multi-modal canonical correlations (CAMCCs) method, which constructs a unified optimization model of multi-modal correlation learning and clustering. The method not only realizes discriminant learning of correlation projection directions under unsupervised cases, but also is able to directly obtain class labels of multi-modal data. Additionally, the method also realizes out-of-sample extension in class labels. Solutions of CAMCCs are optimized by an iterative way, and we analyze its convergence. Extensive experimental results on various datasets have demonstrated the effectiveness of the method. 相似文献
57.
In this paper, a new inverse identification method of constitutive parameters is developed from full kinematic and thermal field measurements. It consists in reconstructing the heat source field from two different approaches by using the heat diffusion equation. The first one requires the temperature field measurement and the value of the thermophysical parameters. The second one is based on the kinematic field measurement and the choice of a thermo-hyperelastic model that contains the parameters to be identified. The identification is carried out at the local scale, ie, at any point of the heat source field, without using the boundary conditions. In the present work, the method is applied to the challenging case of hyperelasticity from a heterogeneous test. Due to large deformations undergone by the rubber specimen tested, a motion compensation technique is developed to plot the kinematic and the thermal fields at the same points before reconstructing the heterogeneous heat source field. In the present case, the constitutive parameter of the Neo-Hookean model has been identified, and its distribution has been characterized with respect to the strain state at the surface of a cross-shaped specimen. 相似文献
58.
Amit Kumar 《热应力杂志》2018,41(8):1080-1099
A unified enriched finite element (FE) formulation for two generalized thermoelsaticity theories is developed for the transient thermal shock problems in one and two dimensional domains. Both the displacement and temperature field interpolations are enriched with harmonic functions defined in the local element coordinates. The coupled field finite element equations are derived using the generalized Hamilton’s principle and solved directly in time domain using the standard Newmark-β time integration technique as opposed to the transform techniques usually adopted for thermal shock problems. The method is assessed in comparison with the Laplace transform based analytical solutions and FE solutions with dynamic meshing available in the literature. It is shown that the present solution with a static uniform mesh captures the sharp discontinuities in the temperature and displacement fields and the wave properties of heat conduction very accurately, practically eliminating the severe drawbacks of the conventional FE solutions such as the spurious undulations and flattening out, while maintaining better computational e?ciency. 相似文献
59.
Airborne spread of expiratory droplet nuclei between the occupants of indoor environments: A review 下载免费PDF全文
This article reviews past studies of airborne transmission between occupants in indoor environments, focusing on the spread of expiratory droplet nuclei from mouth/nose to mouth/nose for non‐specific diseases. Special attention is paid to summarizing what is known about the influential factors, the inappropriate simplifications of the thermofluid boundary conditions of thermal manikins, the challenges facing the available experimental techniques, and the limitations of available evaluation methods. Secondary issues are highlighted, and some new ways to improve our understanding of airborne transmission indoors are provided. The characteristics of airborne spread of expiratory droplet nuclei between occupants, which are influenced correlatively by both environmental and personal factors, were widely revealed under steady‐state conditions. Owing to the different boundary conditions used, some inconsistent findings on specific influential factors have been published. The available instrumentation was too slow to provide accurate concentration profiles for time‐dependent evaluations of events with obvious time characteristics, while computational fluid dynamics (CFD) studies were mainly performed in the framework of inherently steady Reynolds‐averaged Navier‐Stokes modeling. Future research needs in 3 areas are identified: the importance of the direction of indoor airflow patterns, the dynamics of airborne transmission, and the application of CFD simulations. 相似文献
60.
塔径计算是进行三甘醇填料脱水塔设计的首要工作,为了选用准确适用的塔径计算方法,详细介绍了多种塔径计算方法。以两个实际项目的数据为计算案例,分别采用贝恩-霍根(Bain-Haugen)关联式、通用压降关联图法(GPDC)、塔负荷系数法、Aspen HYSYS软件等不同方法计算三甘醇填料脱水塔的塔径,并进行了计算结果偏差分析和计算方法适用性分析。结果分析表明,塔负荷系数法、通用压降关联图法(GPDC)、Aspen HYSYS软件计算结果相近,经圆整后的塔径一致。贝恩-霍根(Bain-Haugen)关联式算出的结果偏差较大,过于保守,分别为11.11%和8.73%。由于通用压降关联图法(GPDC)的计算结果受GPDC关联图内数据点影响准确度不易保证,建议选用塔负荷系数法和Aspen HYSYS软件进行三甘醇脱水填料塔塔径的计算。 相似文献